论文部分内容阅读
目的探讨胃瘦素在幽门螺杆菌(Hp)相关性胃炎中的病理生理学作用。方法根据胃镜和组织学检查诊断为慢性胃炎的61例患者作为研究对象。用Hp-ureA-PCR、14C呼气试验、快速尿速酶试验对受检对象做Hp感染诊断用酶联免疫吸附试验检测Hp感染阳性和阴性患者胃黏膜组织上清液、血清瘦素的水平,用放射免疫实验检测胃黏膜组织上清液IL-1β、IL-1Ra的水平。结果其中Hp感染阳性的慢性胃炎患者31例,Hp感染阴性的慢性胃炎患者30例。Hp感染阳性组胃瘦素水平明显高于Hp感染阴性组,两组间比较差异有统计学意义(P<0.01),血清瘦素水平两组间比较差异无统计学意义(P>0.05);胃组织上清液IL-1β水平两组间比较差异无统计学意义(P>0.05)。相关性分析显示Hp感染阳性组胃瘦素与IL1β显著负相关(r=-0.78,P<0.01)。结论胃瘦素可能通过自分泌或旁分泌作用局部影响胃的功能,同时可能通过调节IL-1β/IL-1Ra的平衡而在Hp相关性胃炎的发生发展及转归中起作用。
Objective To investigate the pathophysiological role of gastric leptin in Helicobacter pylori (Hp) -related gastritis. Methods According to gastroscopy and histological examination of 61 patients diagnosed as chronic gastritis as the research object. Hp-ureA-PCR, 14C breath test, rapid urinary enzyme test on subjects Hp infection diagnosis by enzyme-linked immunosorbent assay Hp infection-positive and negative gastric mucosal tissue supernatant, serum leptin levels The levels of IL-1β and IL-1Ra in gastric mucosal supernatant were detected by radioimmunoassay. Results There were 31 chronic Hp-positive chronic gastritis patients and 30 chronic Hp-negative chronic gastritis patients. The level of gastric leptin in Hp - positive group was significantly higher than that in Hp - negative group (P <0.01). There was no significant difference in serum leptin level between the two groups (P> 0.05). There was no significant difference between the two groups in the level of IL-1β in gastric tissue supernatant (P> 0.05). Correlation analysis showed that there was a significant negative correlation between gastric leptin and IL1β in Hp-positive group (r = -0.78, P <0.01). Conclusions Gastric leptin may affect gastric function locally by autocrine or paracrine action and may play a role in the development and prognosis of Hp-related gastritis by regulating the balance of IL-1β / IL-1Ra.