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作者对居住在喀麦隆盘尾丝虫病高度流行区15年以上的3种不同临床类型的30例病例(盘尾丝虫抗原皮肤划痕试验均呈阳性反应),以ELISA法测定血清中特异性抗体,用免疫扩散法测定总免疫球蛋白量,并作白细胞粘附测定,该法是在平底微滴定孔内进行,每孔加入4～5条幼虫,再加100μl未经稀释的病人血清和100μl养液(内含5×10~3白细胞),另以高免病人血清和正常人血清作对照,置37℃孵育,于1、3.5、24小时在倒置显微镜下观察,计出粘附于幼虫的细胞
The author of 30 cases of three different clinical types living in the highly endemic area of Onchocerciasis in Cameroon for more than 15 years (skin test onchocerciasis eggs were positive), ELISA was used to measure the serum specificity Antibody, total immunoglobulin content by immunodiffusion and white blood cell adhesion assay, the method is carried out in flat-bottomed microtiter wells, adding 4 to 5 larvae per well, plus 100μl of undiluted patient serum and 100μl nutrient solution (containing 5 × 10 ~ 3 leukocytes), the other with high immunity patient serum and normal human serum as a control, incubated at 37 ℃, at 1,3.5,24 hours under an inverted microscope to observe adhesion to Larvae cells