建立了反相高效液相色谱法测定血液中青霉素类药物。０．５ｍｌ血液中，加入甲砜霉素作为内标物，经乙腈沉淀蛋白后，上清液直接进样分析。五种青霉素类药物的线性良好，ｒ为０．９９１８～０．９９９６；相对回收率为９１．７５％～１０７．３３％；变异系数为３．５６％～９．８５％。考察了保存温度对药物分解的影响以及药物在动物血液中消除时限，排除了其它抗生素的干扰 A reversed-phase high performance liquid chromatography was developed for the determination of penicillin in the blood. 0.5ml of blood, adding thiamphenicol as an internal standard, after precipitation of protein by acetonitrile, the supernatant directly injected analysis. The five penicillins showed good linearity, with r ranging from 0.9918 to 0.9996. The relative recoveries ranged from 91.75% to 107.33%. The coefficients of variation ranged from 3.56% to 9.85%. The effect of storage temperature on the decomposition of the drug as well as the elimination of the drug in the animal’s blood and the elimination of interference from other antibiotics