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以江西铅山红芽芋试管苗为材料,探讨不同取材部位、不同预处理剂、不同酸解离时间和不同改良卡宝品红染色时间对江西铅山红芽芋试管苗染色体制片的影响,以期为江西铅山红芽芋的起源、演化及遗传育种提供一定理论依据。结果表明,切取江西铅山红芽芋试管苗根尖,在室温下用0.1%秋水仙素+0.002mol/L 8-羟基喹啉混合溶液预处理4h,然后在60℃条件下用1 mol/L盐酸解离15 min,最后用改良卡宝品红染色5 min,此时染色体制片效果最佳。
The effects of different materials, different pretreatment agents, different time of acid dissociation and different modified card-treble malachite on the chromosome preparation of the test tube of Red Yam in Jiangxi Province were studied by using the test-tube plantlet of Red Yam in Jiangxi Province. , With a view to provide a theoretical basis for the origin, evolution and genetic breeding of the red bud taro in Jiangxi. The results showed that the root tip of the red bud of Jiangxi Yushan red bud was cut and pretreated with 0.1% colchicine + 0.002 mol / L 8-hydroxyquinoline mixed solution at room temperature for 4 h, then incubated at 1 mol / L hydrochloric acid dissociation 15 min, and finally modified card treasure magenta staining 5 min, this time the best chromosome production.