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目的：测定糖尿病大鼠血中内源性ＮＯ合酶抑制物二甲基精氨酸（ＤＭＡ）的含量．方法：在链佐星诱发的糖尿病大鼠测定血清ＤＭＡ的含量和乙酰胆碱（ＡＣｈ）诱导血管内皮依赖性舒张．结果：与对照组相比，糖尿病大鼠ＤＭＡ血清浓度显著增加（５４±１０ｖｓ０７±０３μｍｏｌ·Ｌ－１，Ｐ＜００１）；丙二醛含量也高于对照组（２５±０３ｖｓ１５±０１μｍｏｌ·Ｌ－１，Ｐ＜００１）；糖尿病大鼠ＡＣｈ舒血管效应减弱，其作用可被左旋精氨酸所改善．结论：链佐星诱发糖尿病大鼠高血糖症引起内源性ＤＭＡ含量升高，同时血管内皮依赖性舒张功能被削弱．
Objective: To determine the content of dimethylarginine (DMA), an endogenous NO synthase inhibitor, in blood of diabetic rats. Methods: The streptozotocin-induced diabetic rats were assayed for serum DMA content and acetylcholine (ACh) -induced endothelium-dependent relaxation. Results: Compared with the control group, the concentration of DMA in diabetic rats increased significantly (54 ± 10 vs07 ± 03 μmol·L-1, P <001), and the content of malondialdehyde was also higher than that of the control Group (2.5 ± 03vs15 ± 01μmol·L-1, P <001). The vasodilator effect of ACh in diabetic rats was weakened, and its effect could be ameliorated by L-arginine. CONCLUSIONS: Streptozocin induced hyperglycemia in diabetic rats resulted in increased endogenous DMA content and impaired endothelium-dependent vasodilation.