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目的 :探讨睫状神经营养因子(CNTF)对反应性胶质化的影响。方法 :应用大鼠脑顶叶刺伤模型 ,将动物分为3组。对照组 :刺伤后即原位注射生理盐水10μl;小剂量组 :刺伤后即原位注射0.1g/LCNTF1μg;大剂量组 :刺伤后即原位注射1g/LCNTF10μg ,分别于伤后1、2、7周灌注活杀大鼠 ,胶质纤维酸性蛋白(GFAP)免疫组织化学染色。结果 :对照组1周时伤道及附近GFAP染色增强 ,星形胶质细胞数目和突起数及突起长度增加 ,胞体增大 ,胞浆丰富 ;2周时 ,上述变化仍明显 ,开始形成胶质瘢痕 ;7周时有较明显的胶质瘢痕形成 ;给予CNTF各时间点上述变化均较对照组明显 ,大剂量组胶质变化较小剂量组更显著。结论 :CNTF具有促进胶质化形成的作用。
Objective: To investigate the effect of ciliary neurotrophic factor (CNTF) on reactive gliosis. Methods: The rat parietal lobe stab wounds were used to divide the animals into three groups. In the control group, saline was injected in situ 10μl after injection. Small dose group: 0.1μg / LCNTF 1μg was injected in situ after the puncture. The high dose group: 1μg / LCNTF 10μg After 2, 7 weeks, the rats were sacrificed and the glial fibrillary acidic protein (GFAP) was immunohistochemically stained. Results: In the control group, the GFAP staining in the injured area and nearby area was enhanced. The number of astrocytes, the number of protuberances and the length of protuberances were increased, the cell bodies were enlarged and the cytoplasm was rich. At 2 weeks, the above changes were still obvious, Scars. At 7 weeks, there was more obvious glial scar formation. Compared with the control group, the above changes of CNTF at each time point were obvious. The glial changes of the high dose group were more significant than those of the lower dose group. Conclusion: CNTF can promote the formation of glia.