An aerobic, Gram-negative bacterium, strain 2-5~T, was isolated from a crude oil-contaminated marine sponge collected near Dalian Bay, China, and subjected to a polyphasic taxonomic investigation. Cells of strain 2-5~T were non-spore forming, non-motile, rods 0.2–0.3 μm wide and 1.1–1.2μm long. Strain 2-5~T grew well on nutrient agar, ~TSA, R2 A agar and LB agar. Colonies of strain 2-5~T on LB agar were circular, smooth with entire margins, non-transparent and pale yellow after 3 d of incubation at 30℃. Growth of strain 2-5~T occurred in LN medium with 0–6% Na Cl; no growth occurred in the presence of 8.0% NaCl. Strain 2-5~T grew at 15–42℃ and at pH 6.0–8.0. Comparative 16 S r RNA gene sequence analysis showed that strain 2-5~T clustered with the species of the genus Lysobacter. Its closet neighbors were the type strains of Lysobacter concretionis KC~TC 12205~T(97% similarity), Lysobacter arseniciresistens ZS79~T(96%), and Lysobacter defluii APB-9 ~T(96%). ~The value for DNA-DNA relatedness between strain 2-5请下载后查看，本文暂不支持在线获取查看简介。T and L. concretionis KC~TC 12205 ~T was 23%. Branched fatty acids iso-C16: 0, iso-C15: 0, iso-C11: 0 3-OH, iso-C17: 1ω9c and iso-C11: 0 were found to be predominant. ~The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. Strain 2-5~T had a DNA G+C content of 63.8 mol%. On the basis of the phenotypic, chemotaxonomic, DNA-DNA hybridization and phylogenetic data, strain 2-5~T represents a novel species of the genus Lysobacter, for which the name Lysobacter hymeniacidonis sp. nov. is proposed. ~The type strain is 2-5~T(=CGMCC 1.12190 ~T = JCM 18137~T). An aerobic, Gram-negative bacterium, strain 2-5 ~ T, was isolated from a crude oil-contaminated marine sponge collected near Dalian Bay, China, and subjected to a polyphasic taxonomic investigation. Cells of strain 2-5 ~ T were non -spore forming, non-motile, rods 0.2-0.3 μm wide and 1.1-1.2 μm long. Strain 2-5 ~ T grew well on nutrient agar, ~ TSA, R2 A agar and LB agar. Colonies of strain 2-5 ~ Growth on strain 2-5 ~ T occurred in LN medium with 0-6% NaCl; with a non-transparent and pale yellow after 3 d of incubation at 30 ° C Growth on strain 2-5 ~ T occurred in LN medium with 0-6% Na Cl; in the presence of 8.0% NaCl. Strain 2-5 to T grew at 15-42 ° C and at pH 6.0-8.0. Comparative 16 S r RNA gene sequence analysis showed that strain 2-5 ~ T clustered with the species of the genus Lysobacter. Its closet neighbors were the type of Lysobacter concretionis KC ~ TC 12205 ~ T (97% similarity), Lysobacter arseniciresistens ZS79 ~ T (96%), and Lysobacter defluii APB-9 ~ T fo r DNA-DNA relatedness between strain 2-5 Please download to view, this article does not support online access View profile. T and L. concretionis KC ~ TC 12205 ~ T was 23% Branched fatty acids iso-C16: 0 iso-C15: 0 iso- C11: 0 3-OH iso- C17: 1ω9c and iso-C11: 0 Strain 2-5 to T had a DNA G + C content of 63.8 mol%. On the basis of the phenotypic, chemotaxonomic, DNA-DNA hybridization and phylogenetic data, strain 2-5 ~ T represents a novel species of the genus Lysobacter, for which the name Lysobacter hymeniacidonis sp. nov. is proposed. ~ The Type strain is 2-5 ~ T (= CGMCC 1.12190 ~ T = JCM 18137 ~ T).