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灭菌制剂进行灭菌度检查的增菌试验后,阳性菌对照管大多采用煮沸30min 灭菌、放冷加去污粉或洗衣粉后刷洗,再用自来水,纯水冲洗的方法进行处理,这种方法在刷洗过程中易造成试管破损。为了解决这个问题,笔者直接将去污粉或洗衣粉加入水中与阳性菌对照管一齐加热煮沸10～15min,放冷后直接用自来水、纯水冲洗即可。笔者曾将用这种方法处理的阳性菌对照管进行增菌培养,结果为阴性。
Sterilization preparations for sterility test after the enrichment test, the positive bacteria control most of the use of boiling 30min sterilization, put cold add detergent or washing powder after brushing, and then tap water, pure water washing method for processing, which Kind of method in the brushing process can easily lead to tube damage. In order to solve this problem, the author directly to the decontamination powder or detergent into the water with positive bacteria control tube together boil 10 ~ 15min, let cool directly with tap water, pure water rinse. I have used this method to deal with positive bacteria control tube enrichment culture, the result was negative.