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目的探讨阿司匹林对血小板诱导人乳腺癌MCF-7细胞(MCF-7 human breast cancer cells)上皮间质转化和迁移侵袭能力的影响。方法以未经干预的MCF-7细胞为对照组,分别用不同浓度(0.5、2.0 mmol/L)的阿司匹林、花生四烯酸激活的血小板及不同浓度阿司匹林预处理花生四烯酸激活的血小板分别干预MCF-7细胞,采用细胞划痕及Transwell实验检测肿瘤细胞迁移侵袭能力的变化。Western Blot检测肿瘤细胞上皮间质转化标记蛋白E-cadherin及Vimentin的表达。结果活化的血小板处理后与对照组相比MCF-7细胞迁移侵袭能力显著增强(P<0.05),Western Blot显示E-cadherin表达显著减少(P<0.01),Vimentin的表达显著增加(P<0.01)。阿司匹林预处理血小板活化后干预MCF-7细胞,其迁移侵袭能力较活化血小板组显著降低(P<0.05),E-cadherin表达显著增高(P<0.01),Vimentin的表达显著减少(P<0.05)。阿司匹林单独处理后的MCF-7细胞迁移及侵袭能力较对照组无显著变化(P>0.05),E-cadherin及Vimentin的表达亦无显著改变(P>0.05)。结论体外实验结果表明,阿司匹林可通过抗血小板活化,抑制血小板诱导的乳腺癌MCF-7细胞上皮间质转化和迁移侵袭能力。
Objective To investigate the effect of aspirin on platelet-induced epithelial-mesenchymal transition and migration of human breast cancer MCF-7 cells (MCF-7). Methods The untreated MCF-7 cells served as the control group, respectively, with different concentrations (0.5,2.0 mmol / L) of aspirin, arachidonic acid activated platelets and different concentrations of aspirin pretreatment arachidonic acid activated platelets Interfered with MCF-7 cells, cell scratch and Transwell assay were used to detect the changes of tumor cell migration and invasion. Western Blot was used to detect the expression of epithelial-mesenchymal transition marker protein E-cadherin and Vimentin in tumor cells. Results The migration and invasion of MCF-7 cells were significantly enhanced (P <0.05) compared with the control group. Western Blot showed that the expression of E-cadherin was significantly decreased (P <0.01) and the expression of Vimentin was significantly increased (P <0.01 ). After pretreatment with aspirin, the expression of E-cadherin in MCF-7 cells was significantly lower than that in activated platelets (P <0.01), while the expression of Vimentin was significantly decreased (P <0.05) . The migration and invasion ability of MCF-7 cells treated with aspirin alone had no significant changes compared with the control group (P> 0.05). The expression of E-cadherin and Vimentin also did not change significantly (P> 0.05). Conclusion The results of in vitro experiments indicate that aspirin can inhibit the platelet-induced epithelial-mesenchymal transition and migration of breast cancer MCF-7 cells through anti-platelet activation.