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从鼠伤寒沙门氏菌染色体ＤＮＡ中克隆Ⅰ相鞭毛蛋白基因ｆｌｉＣ￣ｉ，并导入大肠杆菌ＬＣ２ａ中表达；用酸降解法提纯表达产物，测得其分子量为４９Ｋｄ，ＰＩ５．２，与野生型鞭毛蛋白相一致；经Ｄｏｔ－ＥＬＩＳＡ证实表达产物中存在Ｈ－１￣ｉ抗原，进一步应用沙门氏菌属特异性单抗ｄｅ７和ＤＤ４，在间接免疫荧光和Ｗｅｓｔｅｒｎｂｌｏｔ试验中证实它们所识别的共同抗原表位分布于鞭毛蛋白上，是由ｆｌｉＣ中一小段核苷酸序列所编码。
The Ⅰ phase flagellin gene fliC ~i was cloned from S. typhimurium chromosomal DNA and introduced into E. coli LC2a for expression. The product was purified by acid degradation method and its molecular weight was 49Kd, PI5.2, and the wild-type flagellin phase H-1 ~ i antigen was confirmed by Dot-ELISA, and Salmonella-specific McAbs de7 and DD4 were further confirmed by indirect immunofluorescence and Western blot analysis. The common antigen epitope recognized by them was distributed in flagella The protein is encoded by a short nucleotide sequence in fliC.