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目的 观察溴氰菊酯(DM)在大鼠大脑皮层和海马组织诱导的脂质过氧化作用。方法 DM3.12 5、12 .5 0 0mg/kg染毒大鼠,测定其大脑皮层和海马组织丙二醛(MDA)水平和总超氧化物歧化酶(T SOD ,包括Mn SOD和CuZn SOD)、过氧化氢酶(CAT)、谷胱甘肽S 转移酶(GST)、谷胱甘肽过氧化物酶(GSH Px)和谷胱甘肽还原酶(GR)活力。脑组织细胞质碎片γ谷氨酰半胱氨酸合成酶(γGCS)活力和GSH含量用OPA柱前衍生反相高效液相色谱荧光法检测。结果 DM染毒5d ,(1) 12 .5 0 0mg/kgDM组大鼠大脑皮层MDA含量高于3.12 5mg/kgDM组,海马MDA含量高于对照组和3.12 5mg/kgDM组。(2 )两组DM染毒大鼠大脑皮层T SOD和CuZn SOD活力均低于对照组,差异均有统计学意义(P <0 .0 1或P <0 .0 5 )。(3) 12 .5 0 0mg/kgDM组大鼠大脑皮层GSH含量高于对照组,海马GSH含量低于对照组和3.12 5mg/kgDM组;3.12 5mg/kgDM组大鼠大脑皮层GR活力[(11.80±5 .15 )U/mgpro]低于对照组和12 .5 0 0mg/kgDM组[(18.98±3.6 8)、(17.35±2 .4 7)U/mgpro],3.12 5、12 .5 0 0mg/kgDM组大鼠海马GR活力[(2 1.4 6±8.89)、(2 1.6 3±4 .92 )U/mgpro]均低于对照组[(31.2 2±6 .97)U/mgpro];12 .5 0 0mg/kgDM组海马γGCS活力[(1.75±0 .6 0 )nmol·mgpro-1·min-1]低于对照组和
Objective To observe the lipid peroxidation induced by deltamethrin (DM) in rat cerebral cortex and hippocampus. Methods The levels of malondialdehyde (MDA) and total superoxide dismutase (T SOD), including Mn SOD and CuZn SOD, in cerebral cortex and hippocampus were measured in DM3.12 5,12. 500 mg / , Catalase (CAT), glutathione S transferase (GST), glutathione peroxidase (GSH Px) and glutathione reductase (GR) activity. The cytoplasm fraction of γ-glutamylcysteine synthetase (γGCS) activity and GSH content in brain tissue were detected by OPA pre-column derivatization reversed-phase high performance liquid chromatography. Results The MDA content in the cerebral cortex of DM rats exposed to DM for 5 days was significantly higher than that of 3.12 5 mg / kgDM rats (P <0.05). The content of MDA in the hippocampus was higher than that of the control group and 3.12 5 mg / kg DM group. (2) The activities of T SOD and CuZn SOD in cerebral cortex of DM-treated rats in both groups were lower than those in control group (P <0.01 or P <0.05). (3) The content of GSH in cerebral cortex of 12. 500 mg / kgDM group was higher than that of the control group, while the content of GSH in the hippocampus was lower than that of the control group and 3.12 5mg / kgDM group ± 5.15) U / mgpro] was lower than that of the control group and 12.500 mg / kgDM group [(18.98 ± 3.6 8), (17.35 ± 2.47) U / mgpro], 3.12 5,12.500 The GR activity of hippocampus in 0mg / kg DM group was significantly lower than that of control group ([(21.46 ± 8.89) vs (21.63 ± 4.92) U / mgpro] The activity of γGCS in the hippocampus of 12. 500 mg / kgDM group was significantly lower than that of the control group ([(1.75 ± 0.60) nmol · mgpro-1 · min-1]