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用５％二甲基亚砜＋１０％葡萄糖或５％二甲基亚砜＋１０％蔗糖作为冰冻保护剂，能有效减轻中华猕猴桃愈伤组织细胞的超低温伤害。以－１℃／ｍｉｎ的速率降温至－６０～－７５℃时，对愈伤组织细胞的伤害作用最小；以大于１６０℃／ｍｉｎ速率快速化冻，愈伤组织细胞的相对存活率最高。愈伤组织细胞伤害的主要温度区域为－２０～－４０℃；－６０～－７５℃为愈伤组织细胞投入液氮时的“安全温度区”，－６０℃和－７５℃分别为“临界上限温度”和“临界下限温度”。
Using 5% dimethylsulfoxide + 10% glucose or 5% dimethylsulfoxide + 10% sucrose as cryoprotectant can effectively reduce the hypothermia injury of Actinidia chinensis callus cells. At the rate of -1 ℃ / min to -60 ~ -75 ℃, the effect of callus on the callus was the least; the rate of more than 160 ℃ / min rapid freezing, the relative survival rate of callus cells highest. The main temperature range of callus cell injury is -20 ~ -40 ℃; -60 ~ -75 ℃ is the “safe temperature zone” when callus cells are filled with liquid nitrogen; -60 ℃ and -75 ℃ are the “critical Upper limit temperature ”and“ lower limit temperature ”.