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哈茨木霉 (Trichodermaharzianum)T2 2菌株已普遍用于防治包括由终极腐霉 (Pythiumultimum)引起的苗病或根腐病在内的各种病害。玉米自交系Mo17种子经T2 2处理后播种在接种腐霉或未接种的田间土壤内 ,5d后取幼苗的根系或幼茎提取蛋白。结果表明 :在接种腐霉菌的土壤内 ,未进行T2 2处理的 5d龄幼苗长势明显比对照差 ,而经T2 2处理的幼苗长势明显比对照好。T2 2和腐霉菌复合处理及T2 2单独处理对幼苗生长影响基本相同。本研究建立了蛋白质提取和双向电泳分离技术。通过双向电泳及相应的分析软件 (PDQuestTM 2 Dsoftware)可将不同处理的幼苗自交系蛋白进行分离。T2 2菌株处理的根系产生 10 4种上游调控蛋白和 16 4种下游调控蛋白 ,T2 2与腐霉菌复合处理可产生 97种上游调控蛋白和 15 0种下游调控蛋白 ,而用腐霉菌单一处理诱导的上游或下游蛋白的数量明显少于上述 2个处理。T2 2或腐霉菌单一或复合处理的根系蛋白质组图谱与空白对照相比差异显著 ,它们与对照的蛋白质组图谱相似系数分别为 0 .72、0 .5 1和0 .4 9;T2 2与腐霉菌分别处理的蛋白质组图谱间也相差明显 ,两者的相似系数仅为 0 .6 5。进一步研究发现 ,T2 2菌体蛋白质组图谱与上述各种处理的蛋白质组图谱相似系数均很低 ,说明各种处理诱导后的幼苗?
Trichoderma harzianum T2 2 strains have been commonly used to control various diseases including seedling disease or root rot caused by Pythium multimum. The maize inbred line Mo17 seeds were treated with T2 2 and sown in the soil inoculated with Pythium or uninoculated field. The roots or young stems of the seedlings were taken for protein extraction after 5 days. The results showed that in the inoculated Pythium in soil, the 5-day-old T2-untreated seedlings grew significantly worse than the control, while T2 2-treated seedlings grew significantly better than the control. The effects of T2 2 and Pythium treatment and T2 2 treatment on seedling growth were basically the same. This study established a protein extraction and two-dimensional electrophoresis separation technology. Different treatments of seedlings inbred lines were separated by two-dimensional electrophoresis and the corresponding analysis software (PDQuest ™ 2 Dsoftware). The roots treated with T2 2 produced 104 upstream regulatory proteins and 16 4 downstream regulatory proteins. The combination of T2 2 and Pythium resulted in 97 upstream regulatory proteins and 150 downstream regulatory proteins, whereas the single treatment with Pythium induced The number of upstream or downstream proteins was significantly less than the above two treatments. T2 2 or Pythium alone or in combination treatment of the root proteome map significantly different compared with the blank control, proteomics map their similarity coefficients were 0.72,0. 51 and 0. 49; T2 2 and Pythium were also significantly different between the proteome map, the similarity coefficient between the two was only 0.665. Further study found that T2 2 bacterial proteome map and the above-mentioned various proteomics map similarity coefficient is very low, indicating that after the treatment of various seedlings?