论文部分内容阅读
目的 :分析CIK细胞在癌症患者外周血与年龄、性别、疾病种类、分期的关系。方法 :采用流式细胞术 (FCM)对 145例实体瘤患者外周血中的CIK细胞 (CD+ 3 CD+ 56)及NK细胞 (CD-3 CD+ 56)进行标记分析。结果 :在中、晚期实体瘤患者中 ,CIK细胞表达呈高水平 ,CIK细胞占外周血淋巴细胞总数的百分比为 (9 4 5± 5 4 4%) ,在早期表达水平不高 ,为 (3 2 2±2 2 9%) ,两组差异有显著性 (P <0 0 1) ;而年龄、性别、疾病种类并不影响其在外周血中的水平。NK细胞水平不如CIK细胞明显 ,两者无相关性 (P >0 0 5 )。结论 :CIK细胞在中、晚期恶性肿瘤外周血中有较高的表达水平 ,发挥其重要的抗肿瘤细胞免疫作用 ,并不受患者的年龄、性别及疾病种类的影响。
OBJECTIVE: To analyze the relationship between CIK cells in peripheral blood of cancer patients and their age, sex, disease type and staging. METHODS: Peripheral blood CIK cells (CD + 3 CD + 56) and NK cells (CD-3 CD + 56) in 145 solid tumors were analyzed by flow cytometry (FCM). Results: CIK cells were highly expressed in patients with advanced solid tumors. The percentage of CIK cells in the total number of peripheral blood lymphocytes was (94.5 ± 5.44)%, which was not high in the early stage (3 2 2 ± 2 2 9%). There was significant difference between the two groups (P <0.01). However, age, sex and disease type did not affect the level in peripheral blood. The level of NK cells was not as significant as CIK cells (P> 0.05). Conclusion: CIK cells have a higher expression level in the peripheral blood of patients with advanced malignant tumors, playing an important role in anti-tumor cell immunity and are not affected by the patient’s age, sex and disease type.