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目的优化小儿咳喘灵口服液的絮凝除杂工艺,保留有效成分,提高药液澄清度。方法针对小儿咳喘灵原药水提液的杂质成分,采用单宁和壳聚糖复配的方法,即在第1和第2阶段快速搅拌开始时分别加入单宁溶液和壳聚糖溶液,并以绿原酸保留率、蛋白质和鞣质去除率以及处理后上清液的浊度为指标,分别考察了絮凝剂配比及用量、絮凝温度、搅拌桨叶端线速度和搅拌时间对絮凝效果的影响。结果通过实验得到最佳絮凝工艺条件:单宁和壳聚糖的用量分别为0.250、0.500g/L,絮凝温度为20~30℃,快搅速度和快搅时间分别为0.262 m/s和1.0 min,第1阶段的慢搅速度和慢搅时间分别为0.131m/s和2.0 min,第2阶段的慢搅速度和慢搅时间分别为0.105 m/s和5.0 min。该条件下绿原酸的保留率为92.6%,蛋白质和鞣质的去除率分别为55.8%、70.0%,处理后药液放置24 h后浊度为4.4 NTU,放置90 d后浊度为4.7 NTU。结论实验表明单宁和壳聚糖复配用于小儿咳喘灵原药提取液能有效提高杂质去除率,同时提高药液的澄清度和稳定性。
Objective To optimize the flocculation and impurity removal process of Xiaoer Kechuanling Oral Liquid, retain the effective components and improve the clarity of the liquid. Methods According to the impurity component of pediatric Cough Ghost Radix et Rhizoma Coptidis, the method of compounding with tannin and chitosan was adopted, that is, adding tannin solution and chitosan solution respectively at the beginning of rapid stirring in the first and second stages Taking chlorogenic acid retention rate, protein and tannin removal rate, and the turbidity of the supernatant after treatment as indices, the effects of flocculant ratio and dosage, flocculation temperature, stirring speed of blade end and stirring time on flocculation influences. Results The optimal flocculation process conditions were as follows: the dosage of tannin and chitosan were 0.250 and 0.500g / L, the flocculation temperature was 20 ~ 30 ℃, the fast stirring speed and fast stirring time were 0.262 m / s and 1.0 min, the slow stirring speed and the slow stirring time of the first stage were 0.131m / s and 2.0min, the slow stirring speed and slow stirring time of the second stage were 0.105 m / s and 5.0 min respectively. Under these conditions, the retention rate of chlorogenic acid was 92.6%, and the removal rates of protein and tannin were 55.8% and 70.0% respectively. The turbidity after treated for 24 h was 4.4 NTU, and after 90 days, the turbidity was 4.7 NTU. Conclusion The experiment shows that the combination of tannin and chitosan can effectively improve the removal rate of impurities and enhance the clarity and stability of liquid medicine.