目的运用RNAi技术,研究使bcr-abl基因沉默后相关基因表达变化。方法针对bcr-abl基因的融合位点设计siRNAs,用脂质体法转染K562细胞,进行细胞计数及流式细胞仪检测,观察干扰效果,应用RT-PCR检测相关基因表达变化。结果转染组与对照组相比,细胞数减少,有明显的细胞凋亡,bcr-abl,N-ras,c-myc基因的mRNA表达量降低。结论运用RNAi技术,可以有效地诱导细胞凋亡,抑制相关基因的表达,为研究CML的发生机制奠定基础。 Objective To study the changes of related genes after bcr-abl gene silencing using RNAi technology. Methods siRNAs were designed according to the fusion site of bcr-abl gene. Lipofectamine 2000 was transfected into K562 cells for cell counting and flow cytometry. The interference effect was observed. The expression of related genes was detected by RT-PCR. Results Compared with the control group, the number of cells in the transfected group was decreased and the apoptosis was obvious. The mRNA expressions of bcr-abl, N-ras and c-myc were decreased. Conclusion The RNAi technique can effectively induce apoptosis and inhibit the expression of related genes, which lays the foundation for studying the mechanism of CML.