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目的:建立复脉灵胶囊中盐酸麻黄碱、盐酸伪麻黄碱的含量测定方法。方法:采用HPLC法,流动相:乙腈-水-磷酸-三乙胺(3:97:0.1:0.1);色谱柱:Aglient TC-C_(18)(250 mm×4.6 mm,5μm);检测波长:205 nm;流速:1.2 ml·min~(-1);柱温:室温结果:盐酸麻黄碱的进样量在0.187～0.937μg范围内具有良好的线性关系(r=0.999 7),平均回收率为99.26%,RSD为2.70%(n=6);盐酸伪麻黄碱的进样量在0.226～1.130μg范围内具有良好的线性关系(r=0.999 7),平均回收率为98.77%,RSD为2.21%(n=6)。结论:该方法操作简便、灵敏度高、结果准确,可作为复脉灵胶囊中盐酸麻黄碱、盐酸伪麻黄碱的含量测定方法。
Objective: To establish a method for the determination of ephedrine hydrochloride and pseudoephedrine hydrochloride in Fumailing Capsules. Methods: The mobile phase was acetonitrile-water-phosphoric acid-triethylamine (3: 97: 0.1: 0.1) and the mobile phase consisted of Aglient TC-C 18 (250 mm × 4.6 mm, 5 μm) : 205 nm; flow rate: 1.2 ml · min -1; column temperature: room temperature Results: The injection volume of ephedrine hydrochloride has a good linear relationship (r = 0.999 7) within the range of 0.187 ~ 0.937 μg (R = 0.999 7), the average recovery was 98.77%, and the RSD was 2.21% (n = 6). The injection rate of pseudoephedrine hydrochloride was 0.226 ~ 1.130μg with a good linear relationship % (n = 6). Conclusion: The method is simple, sensitive and accurate. It can be used as a method for the determination of ephedrine hydrochloride and pseudoephedrine hydrochloride in Fumailing Capsules.