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目的 研究胃癌腹膜播散过程中腹膜乳斑的作用。方法 在 3组实验动物中 ,分别向大鼠腹腔内接种经 5 溴脱氧尿苷 ( 5 bromodeoxyurdine ,5 BrdU)标记的Sy86B裸鼠可移植性人胃癌细胞 ,每毫升数量分别为 1× 1 0 5 个、1× 1 0 6 个和 1× 1 0 7个。应用免疫组织化学染色 ,观察癌细胞的分布。计数网膜乳斑或非乳斑区标记细胞的数量 ,确定癌细胞的分布。结果 在乳斑或非乳斑区平均标记细胞数 3组的比率分别是 1 63∶1 ( 1× 1 0 5 个 /ml组 )、2 80∶1 ( 1× 1 0 6 个 /ml组 )和 697∶1 ( 1× 1 0 7个/ml组 )。在乳斑或非乳斑区标记细胞数比较差异有非常显著性 (P <0 .0 1 )。结论 标记细胞选择性地侵入网膜乳斑。在胃癌腹膜播散的早期 ,癌细胞具有特异地侵入腹膜乳斑的特性
Objective To study the role of peritoneal milk spot in the process of peritoneal dissemination of gastric cancer. METHODS: In three groups of experimental animals, Sy86B nude mice transplantable human gastric cancer cells labeled with 5 bromodeoxyurdine (5 BrdU) were inoculated intraperitoneally to the rats, and the number of cells per ml was 1×10 5 . One, 1×10 6 and 1×10 7 . Immunohistochemical staining was used to observe the distribution of cancer cells. Count the number of omental or non-milky plaque labeled cells to determine the distribution of cancer cells. Results The ratios of the average number of labeled cells in the milk spot or the non-milky plaque region 3 groups were 1 63:1 (1×10 5 cells/ml group) and 2 80:1 (1×10 6 cells/ml group), respectively. And 697:1 (1×10 7/ml groups). There was a significant difference in the number of labeled cells in the milk spot or non-milky spot (P < 0.01). Conclusion Marker cells selectively invade omental milk spots. In the early stage of peritoneal dissemination of gastric cancer, cancer cells have the characteristic of invading the peritoneal milk spot.