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本文从细胞学、ＤＮＡ凝胶电泳、流式细胞术三方面研究促肝细胞生长素（ｐＨＧＦ）在体外对肝癌细胞增殖活性的影响。结果表明：ｐＨＧＦ对肝癌ＢＥＬ－７４０２细胞增殖有抑制作用，并存在剂量和时间相关性。其４８ｈ的半数抑制浓度（ＩＤ５０）为０．３７ｍｇ／ｍｌ±０．０４ｍｇ／ｍｌ。而３７℃灭活的ｐＨＧＦ对ＢＥＬ－７４０２细胞增殖在１５ｈ无抑制作用，在２４和４８ｈ抑制作用很弱（ＩＤ５０＞１．５ｍｇ／ｍｌ）。ＤＮＡ凝胶电泳结果表明，ｐＨＧＦ可诱导ＢＥＬ７４０２细胞产生细胞凋亡（Ａｐｏｐｔｏｓｉｓ）。流式细胞术（ＦＣＭ）结果显示：ｐＨＧＦ抑制ＢＥＬ－７４０２细胞增殖过程是先使细胞停留在Ｇ０／Ｇ１期，继而诱导细胞产生凋亡。后两项结果均显示ｐＨＧＦ对人肝癌细胞凋亡的诱导里时间和剂量相关性。
In this paper, the effect of hepatocyte growth factor (pHGF) on the proliferation of hepatocellular carcinoma cells in vitro was studied from three aspects: cytology, DNA gel electrophoresis, and flow cytometry. The results showed that pHGF had an inhibitory effect on the proliferation of hepatocellular carcinoma BEL-7402 cells, and there was dose and time correlation. Its 48h median inhibitory concentration (ID50) was 0.37 mg/ml ± 0.04 mg/ml. However, inactivated pHGF at 37 °C had no inhibitory effect on proliferation of BEL-7402 cells at 15 h, but the inhibition was weak at 24 and 48 h (ID50>1.5 mg/ml). The result of DNA gel electrophoresis showed that pHGF could induce apoptosis of BEL7402 cells. Flow cytometry (FCM) results showed that: pHGF inhibited the proliferation of BEL-7402 cells is to first make the cells stay in G0/G1 phase, and then induce apoptosis of cells. The latter two results all showed the time and dose-related effects of pHGF on apoptosis of human hepatoma cells.