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目的 比较铝碳酸镁和硫糖铝在不同pH值条件下对胆酸的体外吸附程度。方法 利用胃十二指肠模型 ,在胃液中加入一定比例胆汁配成模拟胆汁返流的胃液。将模拟胃液调节至pH3,pH5 ,pH7,然后分别加入不同剂量铝碳酸镁和硫糖铝 ,37℃水浴孵育 1h。建立HPLC测定胆酸中各成分浓度的方法 ,并计算吸附率。结果 HPLC方法灵敏度高、特异性强。铝碳酸镁对总胆酸及各组分的吸附率均高于硫糖铝。结论 铝碳酸镁的层状网络结构对胆酸的吸附能力较强 ,可能更适用于胆汁返流型的患者
Objective To compare the adsorption of cholic acid in vitro by magnesium aluminum sulfate and sucralfate at different pH values. Methods The gastroduodenal model was used to add a certain proportion of bile in the gastric juice to make the gastric juice simulating bile reflux. The simulated gastric juice was adjusted to pH3, pH5 and pH7, and then different doses of magnesium aluminum carbonate and sucralfate were respectively added and incubated in a 37 ° C water bath for 1 hour. A method for the determination of the concentration of each component in bile acid by HPLC was established and the adsorption rate was calculated. Results HPLC method with high sensitivity and specificity. The adsorption rate of ALA to total cholic acid and its components were higher than that of sucralfate. Conclusions The layered network structure of aluminum magnesium carbonate has a strong adsorption capacity for cholic acid and may be more suitable for patients with bile reflux