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目的应用表面增强拉曼光谱法检测相关样品内百草枯含量,探究大容量全肺灌洗术对肺组织内百草枯清除效能。方法选用SPF级Wistar大鼠,60 mg/kg为染毒剂量,经口一次灌胃染毒制备动物模型,于染毒后相应时间点采血,行大容量全肺灌洗术留取肺泡灌洗液,留取肺组织。应用表面增强拉曼光谱法检测血浆、肺泡灌洗液及肺组织中百草枯含量,并进行统计分析。结果各时间点(1~168 h)血浆、肺泡灌洗液、肺组织匀浆中均检测到百草枯;染毒后1 h血浆、肺泡灌洗液中百草枯含量达峰值(分别是8 926.3±1 586.47和1 573.9±310.13 ng/ml),12 h肺组织中百草枯达峰值(13 642.2±1 242.66 ng/g),15h时肺泡灌洗液中百草枯含量出现第二高峰;各组灌洗侧(左侧)肺组织内百草枯浓度低于未灌洗侧(右侧),清除率为40%±12%。结论大容量全肺灌洗术对肺组织内百草枯有较高的清除效能,可作为清除肺组织内百草枯一项救治措施。
Objective To detect the content of paraquat in related samples by surface-enhanced Raman spectroscopy, and to investigate the paraquat removal efficiency in lung tissue by high-capacity whole-lung lavage. Methods Wistar rats of SPF grade were used in this experiment. 60 mg / kg was used as the dose, and the animals were orally administered by oral gavage once a day. Blood samples were taken from the rats at the corresponding time points after lavage. Allo-pulmonary lavage was performed for alveolar lavage Fluid, lung tissue collection. The content of paraquat in plasma, alveolar lavage fluid and lung tissue was detected by surface enhanced Raman spectroscopy and statistical analysis was made. Results Paraquat was detected in plasma, alveolar lavage fluid and lung homogenate at each time point (1 ~ 168 h). The content of paraquat in plasma and alveolar lavage fluid peaked 1 h after exposure (8 926.3 ± 1 586.47 and 1 573.9 ± 310.13 ng / ml respectively), the peak of paraquat reached the peak at 12 h (13 642.2 ± 1 242.66 ng / g) in lung tissue and the second peak of paraquat content in BALF at 15 h. Paraquat concentrations in the lavage (left) lung tissue were lower than those on the unwashed side (right) with a clearance of 40% ± 12%. Conclusions High-capacity whole-lung lavage has higher clearance effect on paraquat in lung tissue, which can be used as a treatment measure to remove paraquat in lung tissue.