The hydrolysis reaction of (±)-2-acetamido-3-hydroxy-1-(4-nitrophenyl)-1-propanone[(±)-[STHZ]1], an intermediate of chloramphenicol, was studied and three different products were obtained respectively under different reaction conditions. The resolution of hydrolysis product (±)-2-amino-3-hydroxy- 1-(4-nitrophenyl)-1-propanone, an intermediate of chloramphenicol, was studied and three different products were obtained respectively under different reaction conditions. The resolution of hydrolysis product (±)-2-amino-3-hydroxy- 1-(4-nitrophenyl)-1-propanone[(±)-[STHZ]3] was carried out. A process of crystallization-induced asymmetric transformation was observed and up to 76% of the optically pure enantiomer was obtained in the resolution of (±)- was carried out. A process of crystallization-induced asymmetric transformation was observed and up to 76% of the optically pure enantiomer was obtained in the resolution of (±)-3. The hydrolysis reaction of (±) -2-acetamido-3-hydroxy-1- (4-nitrophenyl) -1- propanone [(±) - [STHZ] 1], an intermediate of chloramphenicol, was studied and three different products were The resolution of the hydrolysis product (±) -2-amino-3-hydroxy-1- (4-nitrophenyl) -1-propanone, an intermediate of chloramphenicol, was studied and three different products were obtained The resolution of the hydrolysis product ± ± 2-amino-3-hydroxy-1- (4-nitrophenyl) -1-propanone [(±) - [STHZ] 3] was carried out. A process of crystallization-induced asymmetric transformation was observed and observed to be 76% of the optically pure enantiomer was obtained in the resolution of (±) - was carried out. A process of crystallization-induced asymmetric transformation was observed and up to 76% of the optically pure enantiomer was obtained in the resolution of (±) -3.