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目的探讨尿激酶(urokinase,UK)对大鼠胸膜的成纤维细胞(fibroblasts,FB)分泌转化生长因子-β1(transforming growth factor beta-1,TGF-β1)、血管内皮生长因子(vascular endothelial growth factor,VEGF)、纤溶酶原激活物抑制剂(plasminogen activator inhibitor-1,PAI-1)的影响。方法取健康雄性SD大鼠胸膜,培养、纯化并鉴定FB。设对照组和实验组。对照组分2组:对照0组由不含胸膜FB的40个样本组成,加入无血清RPMI 1640培养液;对照1组由40个胸膜FB样本组成,加入无血清RPMI 1640培养液,培养24h后,用酶联免疫吸附法测定上清液中TGF-β1、PAI-1、VEGF的含量;实验组分为4组,共40个样本,分别加入5000、10000、20000和30000I U/ml的UK,培养24h后取上清液,测定其中TGF-β1、VEGF、PAI-1量。结果对照0组未检测出TGF-β1、PAI-1、VEGF;对照组1胸膜FB分泌TGF-β1、VEGF、PAI-1的量为(3 135.205±390.975)pg/mL;(22.09±7.48)ng/ml;(1.8775±0.39)ng/ml;加入5000～30000I U/ml尿激酶的试验组,TGF-β1、VEGF、PAI-1的浓度与对照1组相比,有明显下降(P<0.05)。结论胸膜成纤维细胞能分泌TGF-β1、PAI-1、VEGF;而尿激酶能抑制其分泌。
Objective To investigate the effects of urokinase on the secretion of transforming growth factor-β1 (TGF-β1) and vascular endothelial growth factor (TGF-β1) in rat pleural fibroblasts , VEGF) and plasminogen activator inhibitor-1 (PAI-1). Methods The healthy adult male Sprague - Dawley rats were cultured, purified and identified as FB. The control group and experimental group. Control group 2: Control group 0 consisted of 40 samples without pleural FB and added serum-free RPMI 1640 medium; control group 1 consisted of 40 pleural FB samples and serum-free RPMI 1640 medium was added. After 24 h incubation The contents of TGF-β1, PAI-1 and VEGF in the supernatant were determined by enzyme-linked immunosorbent assay (ELISA). The experimental group consisted of 4 groups with 40 samples, and 5000, 10000, 20000 and 30000 IU / ml UK After 24h, the supernatant was taken and the amount of TGF-β1, VEGF, PAI-1 was measured. Results TGF-β1, PAI-1 and VEGF were not detected in control group 0, but the levels of TGF-β1, VEGF and PAI-1 secreted by FB in control group 1 were (3 135.205 ± 390.975) pg / mL; (1.8775 ± 0.39) ng / ml; Compared with control group 1, the concentrations of TGF-β1, VEGF and PAI-1 in the experimental group added 5000-30000 IU / ml urokinase significantly decreased (P < 0.05). Conclusion Pleural fibroblasts can secrete TGF-β1, PAI-1 and VEGF, while urokinase can inhibit its secretion.