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本文以胶质银技术对8例正常骨髓及24例小儿急性白血病治疗前及缓解后骨髓进行了核仁形成区嗜银蛋白(AgNOR)定量研究。其中 L_1型9例,L_2型5例,M_2型6例,M_5型4例,缓解后分别为 L_1型6例,L_2型4例,M_2型4例,M_5型0。结果表明:治疗前骨髓细胞 AgNOR 均值明显高于正常对照组(P<0.01～0.001),以 M_5型最高。缓解后(M_5型除外)AgNOR 均值近似正常组(P>0.05),而较治疗前明显下降(P<0.05～0.01)。提示 AgNOR 的变化可反映骨髓细胞的增殖程度,在小儿急性白血病的诊断、病情监测及疗效与预后判断中可作为一项断的指标。
In this paper, AgNOR quantitative analysis of nucleolar organizer region of bone marrow in 8 normal bone marrow and 24 children with acute leukemia before and after remission were performed by colloidal silver technique. There were 9 cases of L_1 type, 5 cases of L_2 type, 6 cases of M_2 type and 4 cases of M_5 type. Among them, 6 cases were L_1 type, 4 cases L_2 type, 4 cases M_2 type, and M_5 type 0. The results showed that the mean AgNOR of bone marrow cells before treatment was significantly higher than that of the normal control group (P <0.01 ~ 0.001), with the highest M_5 type. After remission (except M_5 type), mean of AgNOR was similar to normal group (P> 0.05), but significantly lower than that before treatment (P <0.05 ~ 0.01). It is suggested that the change of AgNOR can reflect the proliferation of bone marrow cells. It can be used as a broken index in diagnosis, condition monitoring, prognosis and prognosis of children with acute leukemia.