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目的:建立补气舒郁颗粒中人参皂苷Rg1、Re的HPLC测定方法。方法:色谱柱:Diamonsi1ODS-C18柱;流动相:乙腈-水,梯度洗脱;流速:1.0mL.min-1;检测波长:203nm;柱温:25℃。结果:人参皂苷Rg1、Re的线性范围分别是0.272～4.08μg(r=0.999 97)、0.289～4.335μg(r=0.999 94),平均加样回收率分别为99.67%、99.73%,RSD分别为2.87%、3.54%。结论:本方法准确、灵敏、重复性好,可用于补气舒郁颗粒的质量控制。
Objective: To establish an HPLC method for determination of ginsenoside Rg1 and Re in Buqi Shuyu Granules. Method: Column: Diamonsi 1 ODS-C18 column; mobile phase: acetonitrile-water, gradient elution; flow rate: 1.0 mL.min-1; detection wavelength: 203 nm; column temperature: 25°C. Results: The linear ranges of ginsenosides Rg1 and Re were 0.272-4.08 μg (r=0.999 97) and 0.289-4.335 μg (r=0.999 94), respectively. The average recoveries were 99.67% and 99.73%, respectively. 2.87%, 3.54%. Conclusion: This method is accurate, sensitive and reproducible, and can be used for quality control of Buqi Shuyu granules.