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目的分析产气肠杆菌携带blaNDM-1质粒的转移性、质粒复制子分型及blaNDM-1的基因环境。方法产气肠杆菌HN-NDM0711为研究对象,接合实验鉴定质粒转移性,PCR方法对质粒复制子分型,染色体步移技术对blaNDM-1上下游测序,基因组序列比对使用BLASTN和BLASTP,注释使用Vector NTI 11.5.1。结果产气肠杆菌HN-NDM0711接合实验阳性,质粒复制子为IncA/C型;blaNDM-1位于不常见的插入序列ISAba14和IS91之间;在blaNDM-1上游出现一个Tn3转座子和I型整合子,整合子上含有一个由庞大镶嵌序列构成的罕见耐药基因盒。结论 IncA/C型质粒pHN-NDM0711携带blaNDM-1及耐药基因盒来源于不同抗菌药物选择压力环境下的基因重组。只有严格控制临床、工业和农业抗菌药物的合理使用才能从源头上减少此类细菌的产生。
Objective To analyze the transferability of plasmid blaNDM-1 carrying Enterobacter aerogenes, the plasmid replicon typing and the gene environment of blaNDM-1. Methods Enterobacter aerogenes HN-NDM0711 was used as the research object, and the conjugation experiments were used to identify the plasmid metastasis. The plasmid replicon typing was performed by PCR. The upstream and downstream of blaNDM-1 was sequenced by chromosome walking technology. The BLASTN and BLASTP were used for genome sequence alignment. Use Vector NTI 11.5.1. The results showed that the Enterobacter aerogenes HN-NDM0711 was positive and the plasmid replicon was IncA / C. BlaNDM-1 was located between the unusual insertions ISAba14 and IS91. A Tn3 transposon and type I Integrator, the integron contains a large mosaic set of rare resistance gene cassette. Conclusion IncA / C plasmid pHN-NDM0711 carries blaNDM-1 and drug-resistant gene cassettes derived from genes recombined under different antibacterial drug selective stress conditions. Only strict control of the rational use of clinical, industrial and agricultural antimicrobials can reduce the origin of such bacteria.