目的　建立高效液相色谱法同时测定维康胶囊中脂溶性维生素A(Vit A),D2(Vit D2),E(Vit E)含量的方法。方法 采用Puritex C18(200 mm×4.6 mm,5μm);甲醇为流动相;流速为1.0 mL·min-1;检测波长为266 nm;柱温为40℃。结果 Vit A,VitD2,Vit E的检测限分别为0.5,0.1,1.5 ng,线性范围分别为0.41~103.50,0.14~35.60,2.00~500.00μg·mL-1,平均回收率分别为99.89％,99.55％,99.53％。结论　该方法简单、快速、灵敏度高、重复性好。 Objective To establish a method for the simultaneous determination of the contents of Vit A, D2 (Vit D2) and E (Vit E) in Weikang Capsules by high performance liquid chromatography. Methods Puritex C18 (200 mm × 4.6 mm, 5 μm) was used as the mobile phase. The flow rate was 1.0 mL · min-1. The detection wavelength was 266 nm. The column temperature was 40 ℃. Results The detection limits of Vit A, VitD2 and Vit E were 0.5, 0.1 and 1.5 ng, respectively. The linear ranges were 0.41 ~ 103.50, 0.14 ~ 35.60 and 2.00 ~ 500.00 μg · mL -1, respectively. The average recoveries were 99.89% and 99.55 %, 99.53%. Conclusion The method is simple, rapid, sensitive and reproducible.