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目的探讨左旋精氨酸对肺缺血/再灌注损伤(PIRI)时Fas/FasL表达的影响。方法采用在体兔单肺原位缺血/再灌注模型。实验兔30只,随机分为假手术对照组(C组)、肺缺血/再灌注组(I/R组)和肺缺血/再灌注加左旋精氨酸组(L-Arg组),每组10只。分别于再灌注3h取左肺组织,观察Fas/Fas配体(Fas/FasL)mRNA定位表达、凋亡指数(AI)、肺组织湿干质量比(W/D)、肺损伤组织学定量评价指标(IQA)及光镜、电镜下的组织形态学改变。结果L-Arg组Fas/FasL mRNA在肺小动脉内(外)膜、肺小静脉内膜、肺泡上皮及肺支气管上皮呈弱阳性表达,明显低于L/R组(P＜0．05)；AI、W/D和IQA值显著低于L/R组(P＜0．01和P＜0．05)；肺组织形态学异常改变不同程度减轻。结论左旋精氨酸可下调肺组织Fas/FasL mRNA的表达而减轻细胞凋亡,对PIRI发挥积极的防治作用。
Objective To investigate the effect of L-arginine on the expression of Fas / FasL during lung ischemia / reperfusion injury (PIRI). Methods The single-lung ischemia / reperfusion model was established in rabbits. Thirty rabbits were randomly divided into four groups: sham operation control group (C group), lung ischemia / reperfusion group (I / R group) and L-arginine group Each group of 10. Left lung tissues were harvested at 3 hours after reperfusion, and the localization of Fas / Fas ligand (AI), the wet / dry weight ratio (W / D) of lung tissue and histological evaluation of lung injury were observed Indicators (IQA) and light microscopy, electron microscopy histological changes. Results The expression of Fas / FasL mRNA in L-Arg group was significantly lower than that in L / R group (P <0.05) in the inner and outer pulmonary arteries, ; AI, W / D and IQA values were significantly lower than those in L / R group (P <0.01 and P <0.05). The abnormal changes in lung morphology were alleviated to some extent. Conclusion L-arginine can down-regulate the expression of Fas / FasL mRNA in lung tissue and reduce the apoptosis of PIRI cells.