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目的:探讨c-myc反义RNA对人肝癌细胞系HepG2的抑制作用。方法:用四元复合体基因转移系统将重组c-myc反义RNA表达载体pcDNA3.1-as-c-myc体外瞬时转染HepG2受体细胞,3d后流式细胞仪检测肝癌细胞内靶基因表达、细胞凋亡率并分析细胞周期。c-myc反义RNA转染人肝癌细胞HepG2,筛选稳定表达转染质粒的细胞(HepG2/as-c-myc)接种96孔板,绘制生长曲线。结果:c-myc反义RNA可显著降低细胞c-myc蛋白的表达水平,使细胞生长停滞于G0/G1期。稳定表达反义RNA的细胞生长速度与亲本细胞相比明显减慢,有显著性差异。结论:c-myc反义RNA可有效降低人肝癌细胞系HepG2的c-myc蛋白的表达,出现G0/G1期停滞,抑制肝癌细胞的生长增殖。
Objective: To investigate the inhibitory effect of c-myc antisense RNA on human hepatoma cell line HepG2. Methods: HepG2 cells were transiently transfected with pcDNA3.1-as-c-myc recombinant plasmid of c-myc antisense RNA in vitro by quaternary complex gene transfer system. The target genes were detected by flow cytometry Expression, rate of apoptosis and analysis of cell cycle. C-myc antisense RNA was transfected into human hepatoma HepG2 cells. The stable transfected cells (HepG2 / as-c-myc) were seeded into 96-well plates and the growth curve was drawn. Results: The c-myc antisense RNA significantly decreased the expression of c-myc protein and arrested cells in G0 / G1 phase. The growth rate of cells stably expressing antisense RNA was significantly slower than that of the parental cells, and there was a significant difference. Conclusion: The c-myc antisense RNA can effectively reduce the expression of c-myc in human hepatocellular carcinoma cell line HepG2, and arrest in G0 / G1 phase and inhibit the growth and proliferation of hepatocellular carcinoma cell.