Information of Ophiostoma minus in Yunnan of China was provided, which includes the geographical distribution, pine tree hosts, relatedness with bark beetles and temperatures, and the pathogenicity features. O. minus was proved to be a fungus associated with Tomicus piniperda and T. minor , exhibiting the positive correlation between beetle damage level and the fungal occurrence. The blue stain fungus in Yunnan was mainly isolated from Pinus yunnanensis , with a few isolations from P. densata and P. kesiya . Information of Ophiostoma minus in Yunnan of China was provided, which includes the geographical distribution, pine tree hosts, relatedness with bark beetles and temperatures, and the pathogenicity features. O. minus was proved to be a fungus associated with Tomicus piniperda and T. minor exhibiting the positive correlation between beetle damage level and the fungal occurrence. The blue stain fungus in Yunnan was mainly isolated from Pinus yunnanensis, with a few isolations from P. densata and P. kesiya.