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基于植物的过敏性反应机制,构建了PVY NIb基因和来自于细菌Bacillus amy-loliquefaciens的一类RNase基因-Barnase基因的融合基因的植物表达载体。在此表达载体内两基因的拼接处,保留了原来PVY蛋白酶识别PVY NIb和CP蛋白剪切位点的七肽保守序列。通过农杆菌介导获得此融合基因的转基因烟草植株。病毒侵染试验表明,转基因植物在病毒侵染后,发病症状被改变。少部分转融合基因的植株对病毒侵染表现局部抗性。
Based on the plant-based allergic response mechanism, a plant expression vector was constructed for the fusion gene of PVY NIb gene and a class of RNase gene-Barrease gene from Bacillus amy-loliquefaciens. In this expression vector splicing of the two genes, retained the original PVY protease recognition PVY NIb and CP protein cleavage site of the heptapeptide conserved sequence. Transgenic tobacco plants obtained by Agrobacterium tumefaciens-mediated fusion of this gene. Virus infection test showed that the disease symptoms were changed after the transgenic plants infected with the virus. Plants with a small percentage of trans-fusion genes showed partial resistance to viral infection.