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目的:检测口腔白斑和癌组织中Smad4和Bcl-2的表达情况,探讨二者在口腔白斑的癌变过程中的变化及其相互关系。方法:免疫组织化学SABC法检测Smad4蛋白表达情况及Polymer法检测Bcl-2蛋白的表达情况。结果:Smad4蛋白在正常组织、白斑及癌组织中阳性表达率分别是100.0%、92.3%和33.3%,阳性表达差异有统计学意义(P﹤0.01);Bcl-2蛋白在正常组织中、白斑及癌组织中阳性表达率分别是30.0%、53.8%和100.0%,阳性表达差异有统计学意义(P﹤0.01);Smad4蛋白表达与Bcl-2蛋白表达呈负相关(r=-0.854,P<0.01)。结论:Smad4和Bcl-2可能参与了口腔癌变的过程;在鳞癌的发生发展过程中,Smad4缺失表达和Bcl-2的过表达两者可能单独或协同参与。
OBJECTIVE: To detect the expression of Smad4 and Bcl-2 in oral leukoplakia and cancerous tissues and to explore the changes and their correlations between Smad4 and Bcl-2 in the carcinogenesis of oral leukoplakia. Methods: Immunohistochemical SABC method was used to detect Smad4 protein expression and Polymer method to detect Bcl-2 protein expression. Results: The positive expression rate of Smad4 protein in normal tissues, leukoplakia and carcinoma tissues was 100.0%, 92.3% and 33.3%, respectively. The positive expression of Smad4 protein was statistically significant (P <0.01). In normal tissues, (P <0.01). The expression of Smad4 protein was negatively correlated with the expression of Bcl-2 protein (r = -0.854, P <0.01). Conclusion: Smad4 and Bcl-2 may be involved in the process of oral carcinogenesis. Smad4 and Bcl-2 overexpression may be involved in the process of carcinogenesis and progression of squamous cell carcinoma.