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研究待测组分的提取、净化和定性定量的液相色谱条件,建立测定鱼用饲料中的甲基睾丸素(MT)的高效液相色谱检测方法。用95%甲醇来提取样品中的甲基睾丸酮,石油醚脱脂,取部分提取液过中性氧化铝柱净化,采用C18柱,甲醇和水(72:28/V:V)流动相,紫外检测波长为254nm,上高效液相色谱仪分析。检测样品中甲基睾丸素的低限为10mg/kg,当添加质量浓度为2～20mg/kg时,回收率为89.2%～99.2%。
To study the extraction, purification and qualitative and quantitative liquid chromatographic conditions of the tested components, and to establish a HPLC method for the determination of methyl testosterone (MT) in fish feed. Methyl testosterone was extracted with 95% methanol, petroleum ether was degreased, and part of the extract was purified by passing through a neutral alumina column. The mobile phase was C18 column, methanol and water (72:28 / V: V) Wavelength of 254nm, high performance liquid chromatography analysis. The lower limit of methyl testosterone in the test sample is 10mg / kg. When the mass concentration is 2 ~ 20mg / kg, the recovery rate is 89.2% ~ 99.2%.