论文部分内容阅读
目的:观察丁苯酞(NBP)对脑低灌注大鼠学习记忆的改善作用,并探索其生化机制。方法:永久性结扎老龄大鼠双侧颈总动脉3个月,制备脑低灌注模型。实验分4组:假手术组、溶剂对照组、NBP30 mg.kg-1和120 mg.kg-1组。后2组连续给予NBP45 d后,用Morris水迷宫检测大鼠的学习记忆。同时,取脑组织,用生化法检测超氧化物歧化酶(SOD)活力和丙二醛(MDA)含量。结果:在水迷宫定位航行试验中,NBP可以显著缩短脑低灌注大鼠找到安全台的潜伏期。在空间探索试验中,NBP明显增加脑低灌注大鼠在目标象限活动时间的百分比。同时,NBP降低了大鼠脑组织代偿性增高的SOD活力和MDA水平。结论:NBP可以改善脑低灌注大鼠的学习记忆缺陷,其中减轻脑组织氧化应激损伤是重要机制。
Objective: To observe the effect of butylphthalide (NBP) on learning and memory of rats with hypoperfusion after cerebral hypoperfusion and to explore its biochemical mechanism. Methods: Bilateral common carotid arteries were permanently ligated in aged rats for 3 months to prepare hypoperfusion model. The experiment was divided into 4 groups: sham operation group, solvent control group, NBP30 mg.kg-1 and 120 mg.kg-1 group. After two consecutive groups were given NBP for 45 days, Morris water maze was used to detect the learning and memory of rats. At the same time, the brain tissue was taken out and the activity of superoxide dismutase (SOD) and the content of malondialdehyde (MDA) were detected by biochemistry method. Results: In the water maze positioning navigation test, NBP can significantly shorten the latency of finding a safe platform in cerebral hypoperfusion rats. In space exploration experiments, NBP significantly increased the percentage of brain-perfusion rats in the target quadrant. At the same time, NBP decreased the compensatory increase of SOD activity and MDA level in rat brain. Conclusion: NBP can ameliorate learning and memory deficits in rats with hypoperfusion and reduce the oxidative stress in brain tissue.