论文部分内容阅读
目的:研究人参皂苷Rg3通过促进乳腺癌MDA-MB-231细胞人乳腺珠蛋白A(mammaglobin-A,MGBA)的表达从而抑制细胞增殖的作用机制。方法:MTT法和流式细胞术检测5、10、15μg/ml Rg3对乳腺癌细胞增殖和凋亡的影响,Western blotting检测对乳腺癌细胞内MGBA表达的影响;Rg3和siRNA-MGBA单独或联合处理MDA-MB-231细胞,MTT法和流式细胞术检测其对细胞增殖和凋亡的影响,Western blotting检测其对细胞MGBA表达的影响,敏感硫电极法检测其对乳腺癌MDAMB-231细胞H2S分泌的影响。结果:作用细胞48 h后,与对照组相比,5、10、15μg/ml Rg3组MDA-MB-231细胞增殖抑制率明显增高[(18.78±0.82)%、(33.25±1.17)%、(35.11±0.94)%vs(9.72±0.91)%,均P<0.05],Rg3可促进MDA-MB-231细胞的凋亡(P<0.05),也明显增强MDA-MB-231细胞内MGBA蛋白的表达(P<0.05)。与对照组相比,Rg3组MDA-MB-231细胞增殖抑制率明显升高[(30.12±1.01)%vs(10.66±0.59)%,P<0.05],Rg3+siRNA-MGBA组和siRNA-MGBA组MDA-MB-231细胞增殖抑制率明显降低[(6.61±0.63)%、(7.02±0.46)%vs(10.66±0.59)%,均P<0.05];Rg3组MGBA和胱硫醚-γ-裂解酶(cystathionine-γ-lyase,CSE)的表达和H2S的分泌明显增强,而Rg3+siRNA-MGBA组、siRNA-MGBA组明显抑制(均P<0.05)。结论:Rg3可以明显抑制乳腺癌MDA-MB-231细胞的增殖,并促进凋亡,其作用机制可能是通过增强MGBA蛋白的表达并激活H2S/CSE系统得以实现的。
Objective: To study the mechanism of ginsenoside Rg3 inhibiting the proliferation of human breast cancer cell line MDA-MB-231 by inhibiting the expression of mammaglobin-A (MGBA). Methods: The effects of 5, 10, 15μg / ml Rg3 on the proliferation and apoptosis of breast cancer cells were detected by MTT assay and flow cytometry. The effect of Rg3 and siRNA-MGBA alone or in combination MDA-MB-231 cells were treated with MTT assay and flow cytometry (FCM) to detect the effect of MDA-MB-231 cells on the proliferation and apoptosis of MDA-MB-231 cells. Western blotting was used to detect the effect of MGBA on the expression of MGBA. H2S secretion. Results: Compared with the control group, the inhibitory rates of MDA-MB-231 cells in 5, 10, 15μg / ml Rg3 group were significantly higher than those in the control group after 48 h treatment [(18.78 ± 0.82)%, (33.25 ± 1.17)%, 35.11 ± 0.94)% vs (9.72 ± 0.91)%, all P <0.05]. Rg3 could promote the apoptosis of MDA-MB-231 cells (P <0.05) as well as enhance the expression of MGBA in MDA-MB-231 cells Expression (P <0.05). Compared with control group, the inhibitory rate of proliferation of MDA-MB-231 cells in Rg3 group was significantly higher than that of control group [(30.12 ± 1.01)% vs (10.66 ± 0.59)%, P <0.05] (6.61 ± 0.63)%, (7.02 ± 0.46)% vs (10.66 ± 0.59)%, all P <0.05]. The inhibitory rates of MGBA and cystathionine-γ- The expression of cystathionine-γ-lyase (CSE) and the secretion of H2S were significantly increased, while those of Rg3 + siRNA-MGBA group and siRNA-MGBA group were significantly inhibited (all P <0.05). Conclusion: Rg3 can significantly inhibit the proliferation and promote the apoptosis of breast cancer MDA-MB-231 cells. The mechanism may be through enhancing the expression of MGBA protein and activating the H2S / CSE system.