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稻瘟病是世界范围内影响水稻(Oryza sativa)生产的主要病害。抗稻瘟病基因的发掘和育种利用是控制稻瘟病经济、环保的有效措施。为了揭示云南地方水稻品种子预44广谱持久抗瘟机制,利用江南香糯和子预44杂交构建的F7重组自交群体,采用苗期稻瘟病菌自然诱发接种法,通过调查田间抗瘟性表型数据,结合基因型数据对子预44中的数量抗瘟性位点进行了分析。结果表明,在连锁系数(logarithm of odds,LOD)大于2.0的域值上,共检测出13个QTLs,分别位于第1、2、6、8、12号染色体上。不同位点表型贡献值差异较大,范围为5.8%–21.9%,其中8号染色体上标记RM72–RM404之间的QTLs可解释约61.9%的表型变异,很可能为一个主效抗瘟QTL位点。多个位点的主效和微效抗性相结合可能是子预44持久稻瘟病抗性的分子基础。
Rice blast is a worldwide major disease that affects the production of rice (Oryza sativa). Blast resistance gene blasting and breeding is the use of blast control of economic and environmental protection measures. In order to reveal the broad-spectrum and long-lasting anti-blast mechanism of local rice seeds in Yunnan province, F7 recombination selfing population constructed by Jiangnan fragrant waxy rice and sub-pre-44 was used to inoculate naturally-inoculated seedlings of Magnaporthe grisea. Type data, combined with genotype data on the number of pre-44 in the number of blast sites were analyzed. The results showed that 13 QTLs were detected in the logarithm of odds (LOD)> 2.0, which were located on chromosomes 1, 2, 6, 8 and 12, respectively. The phenotypic contributions of different loci differed greatly, ranging from 5.8% to 21.9%. QTLs between RM72 and RM404 on chromosome 8 accounted for about 61.9% of the phenotypic variation, which is likely to be a major anti-blast QTL locus. The combination of major and minor resistances at multiple loci may be the molecular basis for persistent blast resistance.