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目的建立全血中铬元素的电感耦合等离子体-质谱(ICP-MS)测定方法。方法全血样品经体积分数为0.50%氨水-体积分数为0.01%曲拉通X-100溶液稀释10倍后,直接用ICP-MS法进行分析,以钇元素为内标,并采用碰撞/反应池技术消除质谱干扰。结果方法的测定范围为0.60~10.00μg/L,相关系数为0.999 9,检出限为0.18μg/L,定量下限为0.60μg/L。平均回收率为83.02%~98.30%,批内、批间精密度均小于5.00%,样品在-20℃冰箱中至少可保存7 d。标准物质的测定结果为0.72μg/L,在正常参考值范围内。结论该方法各项指标均满足GBZ/T 210.5—2008《职业卫生标准制定指南第5部分:生物材料中化学物质的测定方法》的要求,适用于全血中铬元素的检测。
Objective To establish a method for determination of chromium in whole blood by inductively coupled plasma mass spectrometry (ICP-MS). Methods Whole blood samples were directly analyzed by ICP-MS after the volume fraction of 0.50% ammonia water-volume fraction of 0.01% Triton X-100 solution was 10 times, the internal standard was yttrium, and the collision / reaction Pool Technology Eliminates Mass Spectrometry Interference. Results The determination range of the method was 0.60 ~ 10.00μg / L, the correlation coefficient was 0.999 9, the detection limit was 0.18μg / L and the limit of quantitation was 0.60μg / L. The average recoveries ranged from 83.02% to 98.30%. The intra- and inter-batch precision were less than 5.00%. Samples were stored for at least 7 days at -20 ° C in the refrigerator. The standard substance was determined to be 0.72 μg / L within the normal reference range. Conclusion The indexes of this method all meet the requirements of GBZ / T 210.5-2008 “Occupational Health Standard Setting Guide Part 5: Determination of Chemical Substances in Biological Materials” and are applicable to the detection of chromium in whole blood.