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旨在保护贵州育种单位知识产权,指导当地甘蔗杂交育种亲本组合的制定。采用SSR标记和毛细管电泳/荧光检测技术相结合(SSR-CE/FD)的技术,对12个贵州甘蔗材料进行基因型分析,利用NTsys软件进行数据处理。21对多态性高的SSR引物共获得131条扩增带,多态性条带比例为90.1%,平均每对引物产生6.2个条带。其中11个条带为7个品种(系)中某一品种(系)的特征条带;利用这些特征条带可以迅速将该7个品种(系)的中某一品种(系)与其他材料区分开。引物SMC31CUQ多态性条带比例为100%,且可以将12个甘蔗材料完全区分开,是分辨率最高的引物。UPGMA聚类分析表明12个甘蔗无性系间的遗传相似系数变异范围为0.64~0.92。本研究成功构建了贵州3个审定品种和9个区试材料基于131个SSR条带的指纹图谱,12个甘蔗无性系之间遗传基础较为狭窄,育种工作中应选用遗传背景差异大的甘蔗亲本,从而拓宽贵州甘蔗品种(系)的遗传基础。
The purpose is to protect the intellectual property rights of Guizhou breeding units and to guide the formulation of the local parental combinations of sugarcane cross breeding. SSR markers and capillary electrophoresis / fluorescence detection technology (SSR-CE / FD) were used to analyze the genotypes of 12 Guizhou sugarcane materials, and NTsys software was used to process the data. Twenty-one pairs of SSR primers with high polymorphism were obtained with 131 bands. The percentage of polymorphic bands was 90.1%, with an average of 6.2 bands per pair of primers. Eleven of them were characteristic bands of one of seven varieties (lines). Using these characteristic bands, one of the seven varieties (lines) could be promptly combined with other materials differentiate. Primer SMC31CUQ polymorphic band ratio of 100%, and 12 sugarcane materials can be completely separate, is the highest resolution primer. UPGMA cluster analysis showed that the genetic similarity coefficient of 12 sugarcane clones varied from 0.64 to 0.92. In this study, we successfully constructed the fingerprinting based on 131 SSR bands of 3 validated cultivars and 9 cultivars in Guizhou Province. The genetic basis of 12 sugarcane clones was relatively narrow. In the breeding work, sugarcane parents with large genetic backgrounds should be selected , So as to broaden the genetic basis of Guizhou sugarcane varieties (lines).