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本文报道对1983—1999年间收集的100株MRSA菌株,以Mu3(hVRSA)和Mu50(VRSA)株作为质控菌株,采用常规E试验法、琼脂稀释法、宏量E试验法、Hiramatsu筛选法、梯度平皿法和改良的PAP法进行hVRSA和VRSA的检测。Hiramatsu筛选法显示100株MRSA中有5%的菌株鉴定为VRSA,有5%的菌株鉴定为
In this paper, 100 strains of MRSA were collected from 1983 to 1999. Mu3 (hVRSA) and Mu50 (VRSA) strains were used as control strains. The routine E test, agar dilution, macrophage E test, Hiramatsu screening, Gradient plate method and modified PAP method for detection of hVRSA and VRSA. Hiramatsu screening showed that 5% of 100 strains of MRSA were identified as VRSA and 5% of strains were identified as