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为筛选出适宜配制横坑切梢小蠹(Tomicus minor Hartig)引诱剂的有效化合物,测定了横坑切梢小蠹雌、雄成虫对寄主云南松主要挥发性化合物的触角电位(EAG)和行为反应。结果表明:与对照相比,在10 g·L-1浓度刺激下所测试的9种植物挥发物都能引起横坑切梢小蠹雌、雄虫触角的EAG反应,且触角对不同挥发物的EAG反应差异显著(P<0.05),其中雌、雄虫对(+)-α-蒎烯、(+)-柠檬烯、(+)-3-蒈烯、β-蒎烯和异松油烯的EAG反应较强;在剂量反应试验中,雌、雄虫的EAG反应都随着(+)-α-蒎烯、(+)-柠檬烯、(+)-3-蒈烯和β-蒎烯浓度的增加而增强,当浓度增加到10 g·L-1时,EAG反应最强;而雌、雄虫对异松油烯的EAG反应在1 g·L-1最强;除(+)-柠檬烯没有明显的定向作用外,(+)-α-蒎烯、β-蒎烯、(+)-3-蒈烯和异松油烯对横坑切梢小蠹均表现出了明显的引诱作用;行为反应多重比较表明,(+)-α-蒎烯在10 g·L-1浓度下对雌雄虫的引诱作用最大。该研究结果可为开发横坑切梢小蠹林间引诱剂提供参考。
In order to screen the effective compound which is suitable for attractant of Tomicus minor Hartig, the tentative potential (EAG) and behavior of male and female adults on the main volatile compounds of Pinus yunnanensis were determined. reaction. The results showed that compared with the control, all the nine plant volatiles tested under 10 g · L -1 stimulated the EAG responses of the female and male antennae, and the effects of different antennae on the volatiles (+) - α-pinene, (+) - limonene, (+) - 3-carene, β-pinene and terpinolene The EAG responses of both male and female were dose dependent with the increase of (+) - α-pinene, (+) - limonene, (+) - camphene and β-pinene EAG was the strongest when the concentration was 10 g · L-1, while the EAG response to terpinolene was the strongest at 1 g · L-1. - (+) - α-pinene, β-pinene, (+) - 3-carene and terpinolene showed obvious attractiveness The results showed that (+) - α-pinene had the most attractive effect on male and female at the concentration of 10 g · L-1. The results of this study can provide a reference for the development of transverse borer Betula angustifolia attractant.