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目的：评价不同浓度七氟醚对大鼠原代皮质神经元的毒性作用。方法出生24 h 内的 SD 大鼠48只,体外培养大鼠皮质神经元并接种于6孔板(2 cm)或12孔板(4.5 mm)培养皿,采用随机数字表法分为四组：1%七氟醚处理组(A 组)、2%七氟醚处理组(B 组)、4%七氟醚处理组(C组)和对照组(D 组),每组12只。A、B、C 组神经元分别给予1%、2%和4%浓度的七氟醚处理6 h,D组神经元正常培养,采用 CCK-8法测定细胞存活率,采用乳酸脱氢酶(LDH)法测定细胞死亡率,采用免疫蛋白印迹(Western blot)法测定凋亡蛋白 caspase-317kDa 片段评价细胞凋亡和促凋亡蛋白Bid、Bim 和 Puma 的表达。结果 B、C 组神经元的存活率明显低于 D 组,死亡率明显高于 D 组, caspase-3的17kDa 片段、Bid、Bim 和 Puma 表达明显高于 D 组(P <0.05)；C 组神经元的存活率明显低于 B 组,死亡率明显高于 B 组,caspase-3的17kDa 片段、Bid、Bim 和 Puma 表达明显高于 B 组(P<0.05)。A、D 组各指标差异均无统计学意义。结论随着吸入七氟醚浓度的升高,七氟醚可能通过促进促凋亡蛋白 Bid、Bim 和 Puma 的表达增加对神经元的毒性作用。“,”Objective To explore the relationship of sevoflurane neurotoxicity with the expres-sion of Bid,Bim,Puma.Methods The cortical neuron from newborn SD rat (within 24 h)were see-ded in 6 or 12 well plate,and then randomly divided into 4 groups.Rat culture cortical neurons in vitro exposed in 1%,2%,4% and 0% sevoflurane for 6h were divided into A,B,C and D group. The effect of neuron viability,death and apoptosis were assessed using CCK-8,LDH and caspase-3 cleavage 1 7kDa expression assay.The expressions of Bid,Bim and Puma were assessed by western blot.Results Compared with group D, there were significant increases of neuron death and apoptosis,but a decrease of neuron viability,and upregulated expressions of Bid,Bim and Puma in group B (P <0.05);Compared with group B,Group C had increased death and apoptosis and de-creased viability of neurons,as well as upregulated expressions of Bid,Bim and Puma (P <0.05 ). Conclusion Along with the increase of the concentration,sevoflurane neurotoxicity was increased by upregulation of Bid,Bim,Puma expression.