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对四川葡萄主产区病毒病的发生和危害状况进行田间普查,并采集了48份葡萄枝条样本进行病毒病的血清学检测和鉴定。经双抗体和三抗体夹心酶联免疫法测试后,从采自四川的这批样本中分别检测出了葡萄茎沟病毒(GVA)、葡萄栓皮病毒(GVB)、葡萄扇叶病毒(GFLV)、葡萄斑点病毒(GFkV)、葡萄卷叶病毒-2(GLRaV 2)和葡萄卷叶病毒-3((GLRaV 3)6种危险性的病毒(株系),它们的检出率分别为14 6%、8 3%、10 4%、27 1%、14 6%和20 8%,另外两种靶标病毒(株系)葡萄卷叶病毒-1(GLRaV 1)和南芥菜花叶病毒(ArMV)则未被发现。在四川省内一些老龄葡萄园内,以及部分引进的葡萄新品种上,病毒病的发生和流行极为普遍,并给葡萄的正常生长和生产造成了严重危害。
Field surveys were carried out on the occurrence and damage status of virus diseases in the main producing areas of Sichuan Province. 48 grape bran samples were collected for serological detection and identification of virus diseases. After tested by sandwich ELISA and sandwich ELISA, GVA, GVB and GFLV were detected from these samples collected from Sichuan Province. Six strains of viruses (strains) were identified as GFVV, GLRaV2 and GLRaV3. The detection rates were 14 6% , 83%, 104%, 271%, 14 6% and 20 8% respectively. The other two target viruses, grapevine leafroll virus-1 (GLRaV1) and Arabidopsis mosaic virus Undiscovered. The occurrence and prevalence of viral diseases are prevalent in some aged vineyards in Sichuan province, as well as in some of the new grape varieties introduced, and have caused serious damage to the normal growth and production of grapes.