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目的调查并分析西非马里地区黑人ABO和Rh血型分布情况。方法西非马里医院门诊采样检测血型的随机患者,排除2岁以下儿童,均为西非马里人。ABO血型采用试管法正反定型,Rh血型用试管法做抗-C,抗-c,抗-D,抗-E,抗-e的分型检测,检测出Rh血型抗-D阴性,再次换批号复测,阴性用抗人球蛋白法检测确认。结果西非马里地区黑人ABO血型表型为O(42.38%)>B(27.54%)>A(22.88%)>AB(7.20%),西非马里地区黑人Rh血型表型为cc Dee(65.98%)>cc DE(11.86%)>Cc Dee(11.34%)>ccdee(5.15%)>Cc DE(2.58%)>CCDee(2.06%)>Ccdee(1.03%),基因频率e(0.925 0)>c(0.904 6)>D(0.751 4)>d(0.248 6)>C(0.095 4)>E(0.075 0),未发现CCDE、CCdee、ccd E、CCd E、Ccd E表型。观察值与期望值差异无统计学意义(P>0.05),Hardy-Weinberg吻合度检验良好,证实本调查数据符合群体遗传学中的Hardy-Weinberg定律,结果可靠。结论本次调查结果作为西非马里地区黑人血型基础研究的一部分,为本地区合理高效采供血和规范术前的免疫血液学检测提供理论依据。
Objective To investigate and analyze the distribution of black blood type ABO and Rh in Mali, West Africa. Methods A random sample of blood samples was collected from outpatient clinics in Mali Hospital of West Africa, excluding children under 2 years of age, all of whom were West African Malians. ABO blood type test tube positive and negative stereotypes, Rh blood group test tube method anti-C, anti-c, anti-D, anti-E, anti-e typing test to detect Rh blood group anti-D negative, Batch retest, negative with anti-human globulin test confirmed. Results The ABO blood group phenotype of blacks in Mali, West Africa was O (42.38%)> B (27.54%)> A (22.88%)> AB cc DE (11.86%)> Cc Dee (11.34%)> ccdee (5.15%)> Cc DE 2.58%> CCDee 2.06%> Ccdee 1.09% and gene frequency e 0.925 0> c 0.904 CCDe, CCdee, ccdE, CCdE and CcdE phenotypes were not found. The results were as follows: D (0.751 4)> d (0.248 6)> C (0.095 4)> E (0.075 0). There was no significant difference between the observed value and the expected value (P> 0.05), and the Hardy-Weinberg test showed good agreement. This result confirms that the data accord with Hardy-Weinberg law in population genetics and the results are reliable. Conclusions The findings of this survey, as part of the basic research on black blood type in Mali, West Africa, provide a theoretical basis for the rational and efficient blood supply and standardization of preoperative immunological hematology in the region.