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目的 探讨CD_(44v6)基因表达与胃癌发生及胃癌生物学行为的关系。 方法 应用抗CD_(44v6)蛋白的单克隆抗体,采用免疫组化ABC方法对正常胃粘膜(n=10)、各级胃粘膜异型增生(轻度n=16,中度n=12,重度n=14)、早期胃癌(n=16)及进展期胃癌(n=52)进行研究,并与胃癌类型、大小、有无淋巴结转移等作了比较分析。 结果 正常胃粘膜CD_(44v6)为阴性,随着胃粘膜病变的进展,CD_(44v6)蛋白的表达率逐渐升高,至进展期胃癌,表达率达到顶峰。轻、中、重度异型增生表达率分别为12％,33％,43％;早期胃癌及进展期胃癌的表达率分别为44％和73％。各级异型增生表达率之间的差异无显著性,而进展期胃癌表达率显著高于早期胃癌(P<0.05),淋巴结转移组的表达率显著高于淋巴结未转移组(82％ vs 56％,P<0.05),肠型胃癌的表达率高于弥漫型胃癌(78％ vs 56％,P<0.05),CD_(44v6)蛋白的表达与胃癌肿块大小无相关性。 结论 胃粘膜重度异型增生在CD_(44v6)基因表达上已具有明显的潜在恶性趋势,CD_(44v6)基因表达阳性的胃癌具有更强的浸润及淋巴结转移的能力。
Objective To investigate the relationship between CD44b6 gene expression and gastric carcinogenesis and gastric cancer biological behavior. Methods The monoclonal antibody against CD44 (44v6) protein was used to detect the gastric mucosal dysplasia (n = 10, n = 12, severe n = 14), early gastric cancer (n = 16) and advanced gastric cancer (n = 52), and compared with the type, size and lymph node metastasis of gastric cancer. Results The normal gastric mucosa CD44v6 was negative. With the progress of gastric mucosal lesions, the expression of CD44d6 protein gradually increased, and reached its peak in advanced gastric cancer. The rates of mild, moderate and severe dysplasia were 12%, 33% and 43% respectively. The rates of early gastric cancer and advanced gastric cancer were 44% and 73% respectively. There was no significant difference in the expression rates of dysplasia at all stages, but the expression rate of advanced gastric cancer was significantly higher than that of early stage gastric cancer (P <0.05). The expression rate of lymph node metastasis was significantly higher than that of lymph node metastasis (82% vs 56% , P <0.05). The expression rate of intestinal-type gastric cancer was higher than that of diffuse gastric cancer (78% vs 56%, P <0.05). There was no correlation between the expression of CD44dv and the size of gastric cancer. Conclusions Severe dysplasia of gastric mucosa has obvious potential malignancy in CD44v6 gene expression. The gastric carcinoma with positive expression of CD44b6 gene has stronger invasion and lymph node metastasis.