Jensen和Trager(1977)的恶性疟原虫体外培养方法已广泛采用。一般来说,要采集几毫升含虫静脉血,在培养之前,用RPMI-1640培养液反复洗涤或通过纤维柱的洗脱,再经两次清洗,分离掉白细胞和血浆。本文报告一个建立新的恶性疟原虫体外培养的简单方法。可以省去采血而进行的静脉穿刺及烦琐的洗涤过程。所选病人的原虫率至少达1%,用消毒的巴氏管在无菌操作下,采集病人手指血约 Methods for in vitro culture of P. falciparum by Jensen and Trager (1977) have been widely used. In general, collect a few milliliters of insect venous blood prior to culture, wash repeatedly with RPMI-1640 medium or elute through a fiber column and wash twice to separate white blood cells and plasma. This article reports a simple method of establishing a new P. falciparum in vitro culture. Blood sampling can be dispensed with venipuncture and cumbersome washing procedures. The selected patient’s protozoa rate of at least 1%, with sterile pasteurisation under sterile operation, collecting the patient’s finger blood about