目的:探讨时间分辨荧光分析技术(DELFIA)筛查新生儿先天性甲状腺功能低下的的准确率。方法:回顾性分析我院2008年1月至2013年5月收治的20000例新生儿的临床资料。分别利用酶联免疫吸附法(ELISA)和时间分辨荧光分析技术检测新生儿足跟血中三碘甲腺原氨酸(T3)、四碘甲腺原氨酸(T4)及促甲状腺素(TSH)水平。比较两种检测方法的准确率。结果:DELFIA初次筛查CH的准确性明显高于ELISA。对召回的新生儿进行TSH复测,DELFIA对TSH≥20 m U/L的检测准确率最高,差异具有统计学意义(P<0.05)。结论:DELFIA在筛查先天性甲状腺功能低下中具有较高的应用价值,可作为临床筛查的首选方法。 Objective: To investigate the accuracy of time-resolved fluorescence analysis (DELFIA) screening of congenital hypothyroidism in neonates. Methods: A retrospective analysis of our hospital from January 2008 to May 2013 admitted 20000 cases of newborn clinical data. The levels of triiodothyronine (T3), tetrathionine (T4), and thyrotropin (TSH) in neonatal heel were measured by enzyme-linked immunosorbent assay (ELISA) and time- )Level. Compare the accuracy of the two detection methods. Results: The accuracy of DELFIA screening for CH was significantly higher than that of ELISA. TSH retest of recalled newborns, DELFIA TSH ≥ 20 mU / L, the highest detection accuracy, the difference was statistically significant (P <0.05). Conclusion: DELFIA has high value in the screening of congenital hypothyroidism and can be used as the first choice of clinical screening.