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目的 :了解巢式聚合酶链反应 (NPCR)技术对支气管内膜结核 (EBTB)的诊断价值。方法 :应用巢式聚合酶链反应 (NPCR)对 2 2例支气管内膜活检组织进行结核分支杆菌DNA检测 ,并与病理检查、刷检涂片、支气管镜检后痰涂片结果比较。对照为 2 6例支气管肺癌患者。结果 :2 2例EBTB活检组织病理检查、刷检涂片、支气管镜检“激惹”后痰涂片及NPCR检测阳性率分别为 9%、18%、2 7%和 6 4% ,后者与前三者相比差异有显著性 (P均 <0 .0 1)。 2 2例支气管肺癌患者结核分支杆菌DNA的NPCR均阴性。结论 :巢式PCR技术有利于EBTB患者的诊断 ,尤其对胸片 (或CT)正常、痰菌阴性、支气管内膜活检组织未发现结核特异性病理变化者。
Objective: To understand the value of nested polymerase chain reaction (NPCR) in the diagnosis of endobronchial tuberculosis (EBTB). Methods: Nested polymerase chain reaction (NPCR) was used to detect the DNA of Mycobacterium tuberculosis in 22 cases of bronchial endometrial biopsy tissues. The results were compared with the results of pathological examination, brush smear and bronchoscopy sputum smear. The control was 26 patients with bronchogenic carcinoma. Results: The positive rates of sputum smear and NPCR in 22 cases of EBTB biopsy, brush smear and bronchoscopy “irritation” were 9%, 18%, 27% and 64% Compared with the former three, the difference was significant (P <0.01). All NPC patients with M. tuberculosis DNA in 22 patients with bronchogenic carcinoma were negative for NPCR. Conclusion: Nested PCR is beneficial to the diagnosis of patients with EBTB, especially for those with normal chest X-ray (CT), negative sputum bacterium, and bronchial endometrial biopsy.