豚鼠麻醉后在无菌条件下每耳注射１００μｌ含１×１０８肺炎克雷伯杆菌（ＫＰ）以形成中耳急性感染动物模型。分别于注射后第２４小时、３天、５天和７天取材。用分光光度法测试中耳渗出物蛋白浓度，用免疫转印法测中耳内细菌及内耳组织热休克蛋白（ｈｓｐ）表达谱，并进... Guinea pigs were anesthetized and injected with 100 μl of Klebsiella pneumoniae (KP) containing 1 × 108 per ear under sterile conditions to form an acute infection of the middle ear in an animal model. The animals were harvested 24 hours, 3 days, 5 days and 7 days after injection respectively. The middle ear exudate protein concentration was measured by spectrophotometry, and the heat shock protein (hsp) expression profile of bacteria and inner ear tissue in the middle ear was measured by ...