论文部分内容阅读
目的:探讨黏附分子对血管瘤增殖退化病理生理演变过程的影响及作用机制。方法:应用SABC免疫组化法,检测增殖期血管瘤及退化期血管瘤的细胞间黏附分子-3(Intercellularadhesionmolecule-3,ICAM-3)、CD34和有核细胞表面的组织相容性抗原DR型(Histocompatibilityantigens-DR,HLA-DR)在血管内皮细胞上的表达情况,将2个时期进行比较;同时以血管畸形及正常皮肤为对照。采用行×列表卡方检验进行统计学分析。结果:ICAM-3和CD34均在增殖期血管瘤高表达(分别为26/28和28/28),而退化期低表达或不表达(分别为20/22和20/22),2个时期差异有显著性(P<0.001);而血管畸形和正常皮肤几乎不表达,这与不同时期血管瘤相比差异有显著性(P<0.001);HLA-DR则与血管内皮细胞的高分化阶段密切相关,增殖期基本不表达(4/28),在分化好的退化期高表达(17/22),2个时期差异有显著性(P<0.001)。结论:ICAM-3、CD34可能在血管形成早期发挥作用,介导内皮细胞间黏附,参与血管瘤发病和消退的病理过程。HLA-DR可能与内皮细胞成熟表型的获得及活化状态有关。
Objective: To investigate the effect and mechanism of adhesion molecules on the pathophysiology and evolution of hemangioma proliferation and degeneration. Methods: SABC immunohistochemical method was used to detect the expression of intercellular adhesion molecule-3 (ICAM-3), CD34 and histocompatibility antigen DR on proliferating hemangiomas and degenerative hemangiomas (Histocompatibility antigens-DR, HLA-DR) in vascular endothelial cells, the two periods were compared; the same time, vascular malformations and normal skin as a control. Using row × list chi-square test for statistical analysis. RESULTS: Both ICAM-3 and CD34 were highly expressed in proliferating hemangiomas (26/28 and 28/28, respectively), but not in degenerative stage (20/22 and 20/22, respectively) and 2 periods (P <0.001). However, vascular malformation and normal skin were almost not expressed, which was significantly different from that in different stages of hemangiomas (P <0.001). HLA-DR was associated with a higher differentiation stage of vascular endothelial cells (4/28) in proliferative phase and high expression (17/22) in well-differentiated degenerative stage. There was significant difference between the two periods (P <0.001). Conclusion: ICAM-3 and CD34 may play roles in the early stage of angiogenesis, mediate the adhesion of endothelial cells, and participate in the pathological process of hemangiomas. HLA-DR may be related to the maturation of endothelial phenotypes and the activation status.