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目的:大鼠在低压舱内进行游泳时,皮毛上会附着大量气泡,严重影响对大鼠运动能力的判断。本实验旨在建立低压舱内稳定可靠的大鼠游泳运动模型。方法:SD大鼠分平原组和高原组,每组大鼠又分为A、B、C、D四组。A组大鼠为对照组;B组大鼠在游泳用水中加入去污剂;C组大鼠实验前1天用脱毛剂脱去头部以下被毛;D组大鼠用实验前1天沸腾后自然冷却的水游泳。大鼠分别置于平原和5 000m低压舱内饲养,24h后各组在其饲养环境下进行负重2.5%体重的游泳运动。观察大鼠游泳运动情况,并记录游泳力竭时间。结果:在平原,往游泳用水中加入吐温20(B组)或去除大鼠被毛(C组)可减轻大鼠身上附着的气泡,而在低压舱中使用这两种方法无效。无论是在舱内还是在舱外,使用实验前1天沸腾后自然冷却的水(D组)可完全消除大鼠游泳时皮毛上附着的气泡。D组大鼠在平原力竭时间为(56±11)min,显著长于高原力竭时间(25±17)min。结论:通过实验前煮沸游泳用水,可消除大鼠在低压舱中游泳时皮毛上附着的气泡,建立稳定可靠的低压舱内大鼠游泳运动模型。
OBJECTIVE: When swimming in the low pressure cabin, a large amount of air bubbles are attached to the fur, which seriously affects the judgment of the exercise capacity of the rats. The purpose of this experiment is to establish a stable and reliable rat swimming model in low pressure cabin. Methods: SD rats were divided into plains group and plateau group. Each group of rats was divided into four groups: A, B, C and D. A group of rats as a control group; B group of rats in swimming water added detergent; C group rats 1 day before the experiment with hair removal agent to remove the head below the coat; D group rats with the day before the experiment boiling After the natural cooling water swimming. Rats were placed in the plains and 5 000m low pressure cabin feeding, 24h after each group in its feeding environment for 2.5% weight-bearing swimming. Observe the rat swimming movement, and record swimming time to exhaustion. RESULTS: Addition of Tween 20 (group B) or removal of rat coat (group C) to swimming water in the plains reduced air bubbles attached to the rat, whereas the use of these two methods was ineffective in the low-pressure compartment. Whether it is in a tank or in a tank, the use of water that naturally cools after 1 day of boiling (Group D) completely eliminates bubbles attached to the coat when the rat is swimming. Rats in group D were depleted in the plain (56 ± 11) minutes, significantly longer than those at the time of exhaustion (25 ± 17) min. Conclusion: The boiling water of swimming before experiment can eliminate the bubbles attached to the fur when the rats swim in the low-pressure cabin, and establish a stable and reliable swimming model of rat in low-pressure cabin.